Group of genetic disorders that affect the structure of haemoglobin, resulting in the production of HbS
Aetiology
Genetic - point mutation in codon 6 of the β globin gene that substitutes glutamine to valine producing βS
Pathophysiology
Mutation alters the structure of the resulting Hb → HbS (⍺2βS2)
HbS polymerises if exposed to low oxygen levels for a prolonged period → distorts the red cell, damaging the RBC membrane
Sickle cell trait (HbAS)
One normal, one abnormal β gene
Asymptomatic carrier state
Few clinical features as HbS level too low to polymerase
May sickle in severe hypoxia e.g. high altitude, under anaesthesia
Blood film normal
Mainly HbA, HbS <50%
Sickle cell anaemia (HbSS)
Two abnormal β genes (βS/βS)
HbS >80%, no HbA
Episodes of tissue infarction due to vascular occlusion - sickle crisis
Symptoms depend on site and severity
Pain may be extremely severe
Precipitatnts include hypoxia, dehydration, infection, cold exposure, stress/fatigue
Chronic haemolysis - shortened RBC lifespan
Sequestration of sickled RBCs in liver and spleen
Hyposplenism due to repeated splenic infarcts
Other sickling disorders (sickle cell disease)
Compound heterozygosity for HbS and another β chain mutation, for example:
HbS/β thalassaemia
HbSC disease; milder, but increased risk of thrombosis
Clinical presentation
Clinical features of sickle cell anaemia
Vaso-occlusive crises - acute pain in the hands and feet due to vaso-occlusion of the small vessels
Acute chest sydrome - caused by infection, fat embolism from necrotic bone marrow in a vaso-occlusive crisis or pulmonary infarction due to sequestration of sickle cells
Shortness of breath, chest pain, pyrexia, hypoxia and new chest x-ray changes due to consolidation
Pulmonary hypertension
Anaemia
Splenic sequestration - vaso-occlusion produces an acute painful enlargement of the spleen
Bone marrow aplasia - most commonly follows infection with parvovirus B19, which invades proliferating erythroid progenitors
Investigations
Bloods - FBC, Hb, red cell indices, blood film
High performance liquid chromatography (HPLC) or electophoresis to quantify haemoglobins present